Guide to RNA-seq mapping with TopHat2: example of gene expression in human brain

Analysis of RNA expression is of the most important bioinformatics tasks. However, with RNA-seq many things can go wrong which makes expression analysis very tricky. In this tutorial we provide quite a detailed guide to RNA-seq mapping and explain some of the important factors you need to consider when doing mapping. You are going to touch a fascinating RNA-seq dataset obtained from a human brain tissue and used to study changes in gene expression patterns during aging in human.
https://insidedna.me/tutorials/view/tophat2-analysis-of-rna-expression-is